The A allele of the G20210A SNP in prothrombin (rs1799963) is associated with an increased likelihood in dangerous blood clot formation, where one copy of the A allele increases the normal risk for a blood clot by ~2.5-fold and two copies increases the risk by ~20-fold.
GeneTAG Technology offers a dual-iDDS probe SNP genotyping assay for both alleles. Representative results are shown below.
The local sequence environment of the G/A polymorphism in factor II makes it difficult distinguish between these targets. Our dual-iDDS probe factor II assay uses two different antiprobes to increase the detection specificity.