GeneTAG Technology

DNA-Detection Switch probe technology

Conventional dual-labeled qPCR probes lack error-checking mechanisms and cannot provide multi-temperature single base discrimination. GeneTAG Technology has developed several error-resistant DNA Detection Switch (DDS) probe systems for high-fidelity detection and/or amplification. DDS probes involve the interaction of two labeled polynucleotide components: a fluorescent probe and a competitive, quencher-labeled antiprobe. Probes bind preferentially to their intended targets, turning on signaling, while dissociating from a nearly complementary antiprobe that otherwise turns off signaling. This general signaling mechanism is termed a ′DNA detection switch′.

Our DDS probes are capable of accurate single-base discrimination at annealing temperature ranges of up to 30ºC. This flexibility helps ensure consistency between different users and facilitates assay multiplexing by overcoming the need to optimize multiple assays to perform at the same annealing temperature.

Generic-component qPCR probes

Generic qPCR probes: Universal, Half-Universal, and MacMan

Our Universal, Half-Universal, and MacMan assays use generic components for lost-cost detection. These probe systems employ competitive antiprobes and are most suitable for presence/absence determinations. Our generic probes also enable low-cost screening of target sites during assay optimization.

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Target-specific qPCR probes

Generic-component qPCR probes

Internal DNA-Detection Switch iDDS probes

We have developed three main types of target-specific probe: antiprobe systems. Our ZIPR primer/probes employ antiprobes for accurate target amplification, while our internal DNA-Detection Switch (iDDS) probes and Flip probes detect internal sequences. Several of our iDDS probes can discriminate between 6 closely related targets differing by 1-2 bases.

iDDS probe principle

Custom assays

Generic-component qPCR probes

Custom assays

Custom-designed probes quantitative polymerase chain reaction

GeneTAG Technology offers custom assays for essentially any target of interest. We offer custom-designed assays using iDDS probes (for high specificity), ZIPR probes (for accurate amplification), or Universal probes (for low cost detection or optimization).

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